THE PHOTODYNAMIC INACTIVATION OF PHAGE PRECURSOR BY METHYLENE BLUE

The Photodynamic Inactivation of Phage Precursor by Methylene Blue

Methylene blue added to suspensions of activated staphylococci in amounts sufficient to furnish 1 x 10(5) molecules of dye/bacterium inactivates the phage precursor content of the cells without causing cell death when the mixtures are exposed to strong light of 4000-8000 A. There is a lag phase of approximately 15 minutes in the photodynamic inactivation of phage precursor by methylene blue. Th...

Fast and slow inactivation of sodium channels: effects of photodynamic modification by methylene blue.

Illumination of crayfish giant axons, during internal perfusion with 0.5 mM methylene blue (MB), produces photodynamic effects that include (i) reduction in total sodium conductance, (ii) shifting of the steady-state inactivation curve to the right along the voltage axis, (iii) reduction in the effective valence of steady-state inactivation and, (iv) potentially complete removal of fast inactiv...

Photodynamic inactivation effect of methylene blue and toluidine blue O on ATCC Escherichia coli and clinical isolate of multidrug-resistant Escherichia coli

Influence of multidrug efflux systems on methylene blue-mediated photodynamic inactivation of Candida albicans.

OBJECTIVES To investigate whether the major fungal multidrug efflux systems (MESs) affect the efficiency of methylene blue (MB)-mediated antimicrobial photodynamic inactivation (APDI) in pathogenic fungi and test specific inhibitors of these efflux systems to potentiate APDI. METHODS Candida albicans wild-type and mutants that overexpressed two classes of MESs [ATP-binding cassette (ABC) and ...

Photodynamic inactivation of pseudorabies virus with methylene blue dye, light, and electricity.

Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 X 10(6) 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration...